| 1 | Fern | Arable | An 8-year-old kid. |
| | Mrs. | Arable | Fern's mother. |
| | Johhn | Arable | Fern's father. |
| | Avery | Arable | Fern's older brother. |
| | Wilbur | | Fern's tiny, white pig. |
| 2 | Homer L. | Zuckerman | Fern's uncle. Owner of the farm. |
| | Edith | Zuckerman | Fern's aunt. |
| 3 | The | Goose | The goose Wilbur met at the Zuckerman's farm. |
| | Lurvy | | Homer's hired man. |
| | The | Cows | Wilbur's friends. |
| | The | Horses | Wilbur's friends. |
| | The | Cocker Spaniel | Wilbur's friends. |
| | The | Sheeps | Wilbur's friends. |
| 4 | Templeton | | A rat that lives under Wilbur's trough. |
| | The Oldest | Sheep | Annoyed at Wilbur. |
| | Charlotte | | Wilbur's best friend. A large, grey spider. |
| 6 | Phoe-bee | | A white-throated Sparrow from Boston. |
| | Peabody | | The song Sparrow living in Zuckerman's farm. |
| 8 | Dr. | Dorian | Fern's doctor. |
| 14 | Henry | Fussy | Fern's friend. |
| 17 | Will | | Pontiac car owner. |
| | Uncle | | An enormous pig in the County Fest. |
| 22 | Joy | | Charlotte's spiderlings. |
| | Aranea | | Charlotte's spiderlings. |
| | Nellie | | Charlotte's spiderlings. |
