| | | Book One |
| Peter | Luce | A Sexologist. Cal's doctor. |
| Calliope H. | Stephanides | Narrator and Protagonist. Aka: Cal. |
| Desdemona | Stephanides | Cal's grandmother. |
| Chapter | Eleven | Cal's brother. |
| Theodora Z. | Stephanides | Cal's mother. Lina's daughter. Aka: Tessie. |
| Milton | Stephanides | Cal's father. Tessie's cousin and wife. |
| Peter | Tatakis | The leading member of the debating society. Aka: Uncle Pete. |
| Jackie | Halas | Owner of a souvenir shop. |
| Nishan | Philobosian | The Stephanides's family physician. |
| Michael | Antoniou | Tessie's brother-in-law and ex-fiancé. |
| Father | Gregorios | The priest at the assumption. |
| Zoe Helen | Stephanides | Cal's aunt. Aka: Zo. |
| Sourmelina | Zizmo | Cal's aunt. Lefty and Desdemona's cousin. Aka: Aunt Lina. |
| | |
Hermaphrodite-person having both male & female sex organs. |
| Alexina | Barbin | A famous hermaphrodite. |
| Gottlieb | Gottlich | A famous hermaphrodite. Aka: Marie Rosine. |
| Euphrosyne | Stephanides | Desdemona's mother. |
| Eleutherios | Stephanides | Desdemona's brother and husband. Aka: Lefty. |
| Lucille | Kafkalis | A girl whom Desdemona fixed a date with his brother. |
| Victoria | Pappas | A girl whom Desdemona fixed a date with his brother. |
| General | Hajnestis | The commander-in-chief of the Greek forces in Asia minor. |
| Toukhie | Philobosian | Nishan's wife. |
| Rose | Philobosian | Nishan and Toukhie's daughter. |
| Anita | Philobosian | Nishan and Toukhie's daughter. |
| Karekin | Philobosian | Nishan and Toukhie's son. |
| Stepan | Philobosian | Nishan and Toukhie's son. |
| Kemal | Pasha | A refugee Dr. Philobosian treated. |
| Mr. | Eugenides | The Smyrna merchant. |
| Aristedes | Sterghiades | The Greek Civil Governor. |
| Mrs. | Bidzikian | A woman who sings across the street. |
| Captain | Phillips | The boat captain. |
| Arthur | Maxwell | The Marine Major. |
| Mark | Bristol | An admiral of the U.S. Navy. |
| George | Horton | An American consul. |
| | | Book Two |
| Henry | Ford | Owner of an automobile company. |
| Sophie | Sassoon | A girl who sold the hair of Desdemona. |
| Richard | Nixon | The current U.S. President. |
| Jimmy | Zizmo | Lina's husband. |
| O'Malley | | Lefty's workmate. |
| Father | Styllanopolous | Head priest of assumption Greek orthodox. |
| Melia | Salakas | A woman whose daughter had a disability. |
| Yiorgos | Salakas | Melia's brother. |
| Herbert | Hoover | The 31st U.S. President. |
| Diego | Rivera | A Mexican artist. |
| E.I | Weiss | Manager of the Packard Motor Company. |
| Sister | Wanda | A woman who job interviewed Desdemona. |
| Minister | Fard | A cult leader. |
| Mabel | Reese | Lefty's business associate. |
| Maurice | Plantagenet | A photographer. |
| Robert | Harris | The King of the order of Islam. |
| James | Smith | Robert's victim. |
| Elijah | Muhammad | The supreme minister of the nation of Islam. |
| Julie | Kikuchi | A girl that Cal dated. |
| A.A | Laurie | An Ophthalmologist. |
| Artie | Shaw | A musician. |
| Gus | Vasilakis | Georgia's husband. Lefty's friend. |
| George | Vasilakis | Gus's wife. |
| Gaia | Vasilakis | Gus and Georgia's daughter. A girl who likes Milton. |
| Sophie | Georgopolous | Milton's friend. |
| Mathilda | Livanos | Milton's friend. |
| Mrs. O'Toole | | Owner of a boardinghouse. |
| Michael | Dukakis | A man who was running for President. |
| Mrs. | Tsontakis | Zoe's 93-year-old neighbor. |
| Miss | Barie | Cal's teacher. |
| Al | Kaline | The great first baseman. |
| Eleni | Papanikolas | Milton's employee. A waitress at Milton's restaurant. |
| Jimmy | Papanikolas | Milton's employee. Cal's godfather. |
| Evelyn | Watson | A member of theosophical society. Lina's friend. |
| | | Book Three |
| Rosalee | | The nurse who cleaned Cal when she was a newborn baby. |
| Bart | Skiotis | A man who owns a construction firm. |
| Rufus | | Cal's dog. |
| Willis | | Cal's dog. |
| Morrison | | Cal's new neighbor. |
| Marius | W. C. Grimes. | Cal's neighborhood friend. |
| Edward | Green | A master shoemaker. |
| Mr. | Benz | Cal's neighbor. |
| Phyllis | Benz | Cal's neighbor. |
| Sharon | Stone | A woman who was struck by a sniper's bullet. |
| Carl | Smith | A fireman who was killed by a sniper. |
| George | Romney | A Republican Governor. |
| John | Throckmorton | A General. |
| Jerome | Cavanaugh | The Mayor. |
| Jane | Marsh | Milton's last real estate agent. |
| Harry | Karras | Milton's neighbor who tried to
buy a house in Grosse Pointe. |
| Hudson | Clark | A man who designed the Middlesex. |
| Pete | Savidis | Milton's neighbor. |
| Clementine | Stark | Cal's new friend. |
| Mrs. | Stark | Clementine's mother. |
| Dr. | Cold | One of Desdemona's physicians. |
| Dr. | Katz | One of Desdemona's physicians. |
| Dr. | Tuttlesworth | One of Desdemona's physicians. |
| Georgie | Pappas | Sophie Sassoon's brother. |
| Steve | Munger | Chapter Eleven's best friend. |
| Nelson | Pickett | Cal's new neighbor. |
| Bonnie | Pickett | Nelson's wife. |
| Mizzi | | Cal's new neighbor. |
| Sam | Grossinger | Cal's orthodox jews neighbor. |
| Hettie | Grossinger | Sam's wife. |
| Maxine | Grossinger | Sam and Hettie's only child. |
| Stephen | Roth | A court judge at U.S District Court. |
| S. | Miyamoto | A man who was rejected by Detroit Police Force. |
| Mr. | Nichols | Detroit's police commissioner. |
| Coleman | Young | Detroit's first African-American Mayor. |
| Carol | Horning | Cal's 6th grade classmate. |
| Rebecca | Urbanus | Cal's co-camper. |
| Miss | Grotowski | Cal's teacher. |
| Doctor | Muller | Desdemona's doctor. |
| Thalia | | Desdemona's aunt. |
| Victoria | | Desdemona's aunt. |
| Becky | Amigliato | Cal's hockey teammate. |
| Mrs. | Drexel | A wagon driver. |
| Miss | Baker | The founder of Trombley Elementary. |
| Coach | Stork | A hockey coach. |
| Reetika | Churaswami | Cal's friend. |
| Maria Barbara | Peracchio | Cal's friend. |
| Norma | Abdow | Cal's friend. |
| Tina | Kubek | Cal's friend. |
| Linda | Ramirez | Cal's friend. |
| Miss | Silber | Cal's Latin teacher. |
| Cleo | | Cal's cousin. |
| Helga | | Cal and Tessie's parlorist. |
| Meg | Zemka | Chapter Eleven's girlfriend. |
| Olivia | | Cal's ex-girlfriend. |
| Mr. | Da Silva | Cal's English teacher. |
| Lizzie | Barton | Cal's campmate. |
| Miss | Schuyler | Cal's teacher. |
| Miss | Durell | A transferee from Mrs. Lampe's class. |
| Mrs. | Lampe | One of the teacher at Cal's school. |
| Margaret | Thompson | First in town whose parents owned massaging shower head. |
| Obscure | Object | A girl with whom Cal falls in love with. |
| Dr. | Bauer | Cal's new doctor. |
| Carol | Henkel | A junior who died in a car accident. Deceased. |
| Beulah | | Object's maid. |
| Mrs. | Object | Obscure Object's mother. |
| Jerome | | Object's brother. |
| Renee | Bauer | Object's friend. Dr. Bauer's daughter. |
| Rex | Reese | Carol's ex-boyfriend. |
| Mr. | Burt | A farmer. |
| | | Book Four |
| Hugh | Hefner | An American publisher. |
| Phil | Donahue | A T.V. show host. |
| Lynn | Harris | Hermaphrodite. |
| Lord | Coke | The great British jurist of the 17th Century. |
| Dr. | Winters | Dr. Luce's colleague. |
| Dr. | Craig | Dr. Luce's colleague. |
| Ed | | A barbershop owner. |
| Myron | | A retired parts salesman. A man who treats Cal as a son. |
| Sylvia | | A former social worker. Myron's wife. |
| Scheer | | Cal's new friend. |
| Mayella | | The waitress. |
| Bob | Presto | Cal's new friend and employer. |
| Matt | | Cal's new friend. |
| Mr. | Go | Man who detects the scent of the swimming pool. |
| Wilhemina | | Bob Presto's Namibian girlfriend. |
| Melanie | The Mermaid | Cal's workmate. |
| Zora | | Cal's workmate. |
| Carmen | | A pre-op male to female transsexual. Cal's workmate. |
| Ellie | | Cal's workmate. |