| Oswald |
Avery | American biologist; DNA pioneer |
| Dr. |
Bangham | Rosalind's former professor |
| Mamie |
Bentwich | Rosalind's aunt |
| Norman |
Bentwich | Attorney General of Palestine |
| J.D. |
Bernal | Pioneer of X-ray crystallography |
| John Desmond |
Bernal | Rosalind's new boss. Aka: Sage |
| Dr. |
Boot | Randall's magnetron co-inventor |
| William Henry |
Bragg | Founder of X-ray crystallography |
| Lawrence |
Bragg | Head of Cavendish; X-ray crystallography pioneer |
| Don |
Caspar | Yale biophysicist |
| Francis |
Crick | Cavendish member; molecular biologist |
| Odile |
Crick | Francis Crick's wife |
| Marie |
Curie | Nobel Prize-winning physicist and chemist |
| Pierre |
Curie | Nobel Prize-winning physicist; Marie's husband |
| Simone |
de Beauvoir | French philosopher and feminist |
| Ms. |
Dumas | Rosalind's landlady |
| Paul |
Emmett | American chemist |
| Honor |
Fell | Member of Randall's staff |
| John |
Finch | Rosalind and Aaron's assistant |
| Rosalind |
Franklin | Protagonist; scientist and narrator |
| Jenifer |
Franklin | Rosalind's sister |
| Colin |
Franklin | Rosalind's brother |
| Ellis |
Franklin | Rosalind's father |
| Muriel |
Franklin | Rosalind's mother |
| David |
Franklin | Rosalind's brother |
| Roland |
Franklin | Rosalind's brother |
| Arthur |
Franklin | Rosalind's grandfather |
| Ursula |
Franklin | Rosalind's cousin |
| Cecil |
Franklin | Ellis's brother; Rosalind's uncle |
| Charlotte |
Franklin | Colin's wife |
| Rachel |
Glaeser | Jacques's girlfriend |
| Lawrence |
Goldsbrough | Founder of Goldsbrough Orchestra |
| Arnold |
Goldsbrough | Leonard's co-founder |
| Raymond |
Gosling | Rosalind's new assistant |
| Nannie |
Griffiths | Rosalind's longtime childhood governess |
| Dr. |
Haisinski | Mering's colleague |
| Jean |
Hanson | Member of Randall's staff |
| Louise |
Heller | Rosalind's co-worker |
| Kenneth |
Holmes | Rosalind and Aaron's assistant |
| John |
Kendrew | Rosalind's co-researcher |
| Aaron |
Klug | Rosalind's floormate |
| Dr. |
Linken | Rosalind's surgeon |
| Vittorio |
Luzzati | Rosalind's new workmate |
| Denise |
Luzzati | Vittorio's wife |
| Jacques |
Maire | Rosalind's research student |
| Marcel |
Mathieu | Expert in X-ray crystallography |
| James |
Maxwell | Founder of Cavendish Laboratory |
| Gregor |
Mendel | Founder of modern genetics |
| Jacques |
Mering | Head of the lab |
| Myrtle |
Montefiores-Franklin | David's wife |
| Margaret |
Nance | Rosalind's friend in Paris |
| Professor |
Norrish | Rosalind's professor at Cambridge |
| Laurence |
Olivier | British actor |
| Jan |
Oort | Dutch astronomer |
| Linus |
Pauling | American chemist; Nobel laureate |
| Peter |
Pauling | Linus Pauling's son |
| Jean |
Perrin | French physicist who proved molecules exist |
| Max |
Perutz | Molecular biologist; Nobel laureate |
| John Tutron |
Randall | Head of biophysics research unit |
| Jonas |
Salk | Creator of polio vaccine |
| Jean-Paul |
Sartre | French philosopher and writer |
| David |
Sayre | Rosalind's colleague |
| Anne |
Sayre | Writer; David's wife |
| Erwin |
Schrödinger | Nobel Prize-winning physicist |
| Bill |
Seeds | Wilkins's new workmate |
| Alex |
Stokes | Rosalind's co-worker |
| Freda |
Ticehurst | Laboratory photographer |
| James |
Watson | Newest team member. Aka: Jimmy |
| Adrienne |
Weill | Rosalind's mentor |
| Marianne |
Weill | Adrienne's daughter |
| Maurice |
Wilkins | Assistant director of biophysics unit |
| Ernest |
Woolan | Creator of radiation monitoring device |
| Agnes |
| Rosalind's new researcher |
| Alain |
| Rosalind's workmate |
| Albertine |
| Widow's maid |
| Alice |
| Rosalind's aunt |
| Gabriel |
| Rosalind's workmate |
| Genevieve |
| Rosalind's workmate |
| Hugh |
| Rosalind's uncle |
| Jacob |
| Rosalind's great-grandfather |
| Michel |
| New recruit |
| Rachel |
| Rosalind's new researcher |
| Richard |
| Don's American friend |