| 1 | Mr. | Mole | Protagonist. |
| | The | Rabbit | An elderly rabbit the mole met at the meadow. |
| | Water | Rat | River-banker. Accompanied the mole in his excursion. aka: Ratty. |
| | Mr. | Badger | Kind-hearted animal. Hates society. aka: The Badger. |
| | The | Otter | The rabbit's friend in the river. |
| 2 | The | Duck | Ratty's friend. |
| | Mr. | Toad | Richest, most boastful animal in the entire river. |
| | The | Horse | Old, grey horse. Someone Mr. Mole met at the Paddocks. |
| 4 | Older | Hedgehod | An animal that got lost in the snow. |
| | Little | Billy | Older Hedgehog's small brother. Field mice. |
| 5 | Tommy | | Field mice. |
| | Field | Mouse | The mole's old friend. Tommy and Little Billy's master. |
| 6 | Mr. | Clerk | Imposes an offence to Mr. Toad. |
| | The | Chairman | Chairman of the bench of magistrate. |
| | Police | | Police sergeant. |
| | Oddsbodikins | | Ancient goaler. Jail warden. |
| 7 | The Willow | Wren | Singing animal in the river. |
| | Little | Portly | Otter's son. |
| 8 | Gaoler's | Daughter | Pleasant wench and good-hearted woman. Became Toad's friend. |
| | Washerwoman | | Helped Toad escape from prison. |
| | Engine | Driver | Burly man with an oiled can. Someone Toad met at the prison. |
| 9 | The Third | Swallow | Water Rat's friend. |
| | The | Wayfarer | Seafaring rat from Constantinople. |
| 10 | The Barge | Woman | Big, stout woman Toad met at the canal. |
| | The | Gypsy | Peddler who wants to buy Toad's horse. |
| | The | Driver | Let Toad drive his vehicle. |
| 11 | Weasles | | Wild wooders that took over Toad's hall. |
| | Stoats | | Wild wooders that took over Toad's hall. |
