| | | | Part 1 |
| 1 | Aunt | Vidala | One of the aunts. One of the founders. |
| | | | Aunts - Women assigned to indoctrinate the Handmaids. |
| | | | Handmaids - Fertile women who are forced to bear a child. |
| | Aunt | Helena | One of the aunts. One of the founders. |
| | Aunt | Elizabeth | One of the aunts. One of the founders. |
| | | | Part 2 |
| 1 | | | Ardua Hall - The home of the Aunts. |
| | Aunt | Estee | One of the Aunts. A craft teacher. |
| 2 | Tabitha | | Agnes's adoptive mother. |
| 3 | Commander | Kyle | Agnes's adoptive father. |
| | Agnes | Jemima | One of the narrators. Protagonist. Aka: Aunt Victoria. |
| 4 | | | Marthas - A housekeeper. |
| | Vera | | One of the Marthas of Agnes's family. |
| | Rosa | | One of the Marthas of Agnes's family. |
| | Zilla | | One of the Marthas of Agnes's family. |
| | Becka | Grove | Agnes's classmate. Aka: Aunt Immortelle. |
| | | | Part 4 |
| 7 | Neil | | Daisy's adoptive father. |
| | Melanie | | Daisy's adoptive mother. |
| | Daisy | | One of the narrators and protagonists. Aka: Baby Nicole, Jade. |
| 8 | George | | A homeless man. |
| | Ada | | Melanie's friend. |
| 11 | Aunt | Adrianna | The dead Pearl Girl in Toronto. |
| | | | Part 5 |
| 12 | Commander | Judd | The leader of the Sons of Jacob. |
| | Aunt | Lydia | One of the narrators. Protagonist. |
| | Katie | | One of Aunt Lydia's colleagues. |
| | Anita | | One of Aunt Lydia's colleagues. |
| | Tessa | | The receptionist. |
| 13 | Commander | Saunders | One of the commanders of the Sons of Jacob. |
| | Paula | | Commander Kyle's new wife. Agnes's step-mother. |
| 15 | Ofkyle | | Commander Kyle and Paula's handmaid. |
| 17 | Dr. | Grove | Becka's pervert father. A dentist. |
| | Mr. | William | Dr. Grove's assistant. |
| 18 | Mark | | Commander Kyle and Paula's son. Ofkyle's biological son. |
| | | | Part 7 |
| 20 | Aunt | Clover | One of the Aunts. |
| | | | Part 8 |
| 21 | Elijah | | The deskman at SantuCare. Neil's friend. |
| | | | SantuCare - The Gilead Refugee Organization. |
| | | | Part 10 |
| 25 | Aunt | Gabbana | One of the Aunts. |
| 27 | Aunt | Lise | One of the Aunts. |
| 29 | Cardinal | Newman | The Gilead cardinal. |
| | | | Part 12 |
| 30 | Garth | | Ada and Daisy's transport. |
| | | | Part 14 |
| 36 | Aunt | Lorna | One of the Aunts. A wedding arranger. |
| | Sara | Lee | A wedding arranger. |
| | Betty | | A wedding arranger. |
| 38 | Aunt | Silhoutte | One of the Aunts. Aunt Estee's colleague. |
| | | | Part 16 |
| 44 | Aunt | Beatrice | The Aunts that Daisy met. |
| | Aunt | Dove | The Aunts that Daisy met. |
| | | | Part 24 |
| 63 | Aunt | Clover | Aunt Vidala's gardener. |
| 66 | Captain | Mishimengo | The boat captain. Daisy and Agnes's transport. |
| | | | Part 27 |
| | Mary | | The Queen of Scots. |
